Zebrafish: An In Vivo Screening Model to Study Ocular Phenotypes.

ABSTRACT:

Purpose

To establish a set of assays that allow the in vivo screening of candidate genes for ocular diseases in zebrafish, with an emphasis on refractive error.

Methods

Our pipeline includes the most relevant ocular screening measurements to assess (1) ocular biometry using spectral domain optical coherence tomography, (2) refractive status using an eccentric photorefractor, (3) intraocular pressure by tonometry, and (4) optokinetic response to study visual capability in zebrafish. To validate our pipeline and to demonstrate the potential of zebrafish as a valid animal model, we chose two well-characterized genes with an ocular phenotype (PRSS56 and FBN1) and generated two mutant zebrafish lines (prss56 and fbn1). Mutant fish were assessed at 2, 4, and 6 months after fertili

SUBMITTER: Quint WH 

PROVIDER: S-EPMC8934544 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature