Deciphering the Molecular Signature of Human Hyalocytes in Relation to Other Innate Immune Cell Populations.

ABSTRACT:

Purpose

Hyalocytes are the tissue-resident innate immune cell population of the vitreous body with important functions in health and vitreoretinal disease. The purpose of this study is to gain new insights into the biology and function of human hyalocytes in comparison to other innate immune cells.

Methods

The present study applies fluorescence-activated cell sorting and RNA sequencing to compare the transcriptional profiles of human hyalocytes, retinal microglia (rMG) and classical, intermediate, and non-classical monocytes isolated from the same patients. Immunohistochemistry was applied for morphological characterization of human hyalocytes.

Results

Pairwise analysis indicates distinct differences between hyalocytes and monocytes, whereas a high degree of similarit

SUBMITTER: Wolf J 

PROVIDER: S-EPMC8934546 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature