Sensory neuron transcriptomes reveal complex neuron-specific function and regulation of mec-2/Stomatin splicing.

ABSTRACT: The function and identity of a cell is shaped by transcription factors controlling transcriptional networks, and further shaped by RNA binding proteins controlling post-transcriptional networks. To overcome limitations inherent to analysis of sparse single-cell post-transcriptional data, we leverage the invariant Caenorhabditis elegans cell lineage, isolating thousands of identical neuron types from thousands of isogenic individuals. The resulting deep transcriptomes facilitate splicing network analysis due to increased sequencing depth and uniformity. We focus on mechanosensory touch-neuron splicing regulated by MEC-8/RBPMS. We identify a small MEC-8-regulated network, where MEC-8 establishes touch-neuron isoforms differing from default isoforms found in other cells. MEC-8 establishes the

SUBMITTER: Liang X 

PROVIDER: S-EPMC8934639 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature