Extended sister-chromosome catenation leads to massive reorganization of the E. coli genome.

ABSTRACT: In bacteria, chromosome segregation occurs progressively from the origin to terminus within minutes of replication of each locus. Between replication and segregation, sister loci are held in an apparent cohesive state by topological links. The decatenation activity of topoisomerase IV (Topo IV) is required for segregation of replicated loci, yet little is known about the structuring of the chromosome maintained in a cohesive state. In this work, we investigated chromosome folding in cells with altered decatenation activities. Within minutes after Topo IV inactivation, massive chromosome reorganization occurs, associated with increased in contacts between nearby loci, likely trans-contacts between sister chromatids, and in long-range contacts between the terminus and distant loci. We deciph

SUBMITTER: Conin B 

PROVIDER: S-EPMC8934667 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature