Generation and characterization of human Fetal membrane and Decidual cell lines for reproductive biology experiments†.

ABSTRACT: Human fetal membrane and maternal decidua parietalis form one of the major feto-maternal interfaces during pregnancy. Studies on this feto-maternal interface is limited as several investigators have limited access to the placenta, and experience difficulties to isolate and maintain primary cells. Many cell lines that are currently available do not have the characteristics or properties of their primary cells of origin. Therefore, we created, characterized the immortalized cells from primary isolates from fetal membrane-derived amnion epithelial cells, amnion and chorion mesenchymal cells, chorion trophoblast cells and maternal decidua parietalis cells. Primary cells were isolated from a healthy full-term, not in labor placenta. Primary cells were immortalized using either a HPV16E6E7 retro

SUBMITTER: Radnaa E 

PROVIDER: S-EPMC8934701 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature