Knockdown of XIST up-regulates 263294miR-340-5p to relieve myocardial ischaemia-reperfusion injury via inhibiting cyclin D1.

ABSTRACT:

Aim

Long non-coding RNAs (lncRNAs) are known to participate in various human diseases, while the role of X inactive-specific transcript (XIST) binding microRNA-340-5p (miR-340-5p) remains seldom studied. We aim to identify the role of the XIST/miR-340-5p/cyclin D1 (CCND1) axis in the myocardial ischaemia-reperfusion injury (MIRI).

Methods and results

The mouse MIRI models were established. The expression of XIST, miR-340-5p, and CCND1 in mouse myocardial tissues in MIRI mice was assessed. The MIRI mice were respectively treated with altered XIST, miR-340-5p, or CCND1. The changes of myocardial enzyme activity were assessed, and the cardiac function was evaluated. Myocardial pathological changes, cardiomyocyte apoptosis and related apoptotic factors, oxidative stress and infl

SUBMITTER: Bai Q 

PROVIDER: S-EPMC8934972 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature