A coarse-grained NADH redox model enables inference of subcellular metabolic fluxes from fluorescence lifetime imaging.

ABSTRACT: Mitochondrial metabolism is of central importance to diverse aspects of cell and developmental biology. Defects in mitochondria are associated with many diseases, including cancer, neuropathology, and infertility. Our understanding of mitochondrial metabolism in situ and dysfunction in diseases are limited by the lack of techniques to measure mitochondrial metabolic fluxes with sufficient spatiotemporal resolution. Herein, we developed a new method to infer mitochondrial metabolic fluxes in living cells with subcellular resolution from fluorescence lifetime imaging of NADH. This result is based on the use of a generic coarse-grained NADH redox model. We tested the model in mouse oocytes and human tissue culture cells subject to a wide variety of perturbations by comparing predicted fluxes

SUBMITTER: Yang X 

PROVIDER: S-EPMC8935353 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature