High-throughput estimation of allele frequencies using combined pooled-population sequencing and haplotype-based data processing.

ABSTRACT:

Background

In addition to heterogeneity and artificial selection, natural selection is one of the forces used to combat climate change and improve agrobiodiversity in evolutionary plant breeding. Accurate identification of the specific genomic effects of natural selection will likely accelerate transfer between populations. Thus, insights into changes in allele frequency, adequate population size, gene flow and drift are essential. However, observing such effects often involves a trade-off between costs and resolution when a large sample of genotypes for many loci is analysed. Pool genotyping approaches achieve high resolution and precision in estimating allele frequency when sequence coverage is high. Nevertheless, high-coverage pool sequencing of large genomes is expensive.

Re

SUBMITTER: Schneider M 

PROVIDER: S-EPMC8935755 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature