Genetic control of the dynamic transcriptional response to immune stimuli and glucocorticoids at single cell resolution.

ABSTRACT: Synthetic glucocorticoids, such as dexamethasone, have been used as treatment for many immune conditions, such as asthma and more recently severe COVID-19. Single cell data can capture more fine-grained details on transcriptional variability and dynamics to gain a better understanding of the molecular underpinnings of inter-individual variation in drug response. Here, we used single cell RNA-seq to study the dynamics of the transcriptional response to glucocorticoids in activated Peripheral Blood Mononuclear Cells from 96 African American children. We employed novel statistical approaches to calculate a mean-independent measure of gene expression variability and a measure of transcriptional response pseudotime. Using these approaches, we demonstrated that glucocorticoids reverse the effect

SUBMITTER: Resztak JA 

PROVIDER: S-EPMC8936121 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature