Detection of lymph node metastasis in non-small cell lung cancer using the new system of one-step nucleic acid amplification assay.

ABSTRACT:

Introduction

The prognosis of non-small cell lung cancer greatly depends on the presence of lymph node metastasis, which limits the need for surgery and adjuvant therapy for advanced cancer. One-step nucleic acid amplification of cytokeratin19 (CK19) mRNA was used to detect lymph node metastasis. Automated Gene Amplification Detector RD-200 and the LYNOAMP CK19 gene amplification reagent as components of the new one-step nucleic acid amplification system, which has increased gene amplification efficiency by improving the reagent composition, have shorter preprocessing and measurement times than conventional systems. We aimed to compare the clinical performance of the new system with that of histopathology and the conventional system.

Materials and methods

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SUBMITTER: Ose N 

PROVIDER: S-EPMC8936453 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature