Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.

ABSTRACT:

Background

Integrin family are known as key gears in focal adhesion for triple-negative breast cancer (TNBC) metastasis. However, the integrin independent factor TLN1 remains vague in TNBC.

Methods

Bioinformatics analysis was performed based on TCGA database and Shengjing Hospital cohort. Western blot and RT-PCR were used to detect the expression of TLN1 and integrin pathway in cells. A small-molecule C67399 was screened for blocking TLN1 and integrin β1 through a novel computational screening approach by targeting the protein-protein binding interface. Drug pharmacodynamics were determined through xenograft assay.

Results

Upregulation of TLN1 in TNBC samples correlates with metastasis and worse prognosis. Silencing TLN1 in TNBC cells significantly attenuated t

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8937232 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature