A protocol to differentiate nociceptors, mechanoreceptors, and proprioceptors from human pluripotent stem cells.

ABSTRACT: Human pluripotent stem cells (hPSCs) show promise for studying diseases affecting cell populations that are not easily available, including sensory neurons (SNs). Here, we present a differentiation protocol in chemically defined conditions to generate peripheral SNs from hPSCs. We describe four main steps: expansion of hPSCs, neural crest cell (NCC) differentiation, NCC dissociation and replating, and sensory neuron (SN) differentiation. This protocol enables generation of a mechanoreceptor-enriched culture or a population containing all three SN subtypes (nociceptors, mechanoreceptors, and proprioceptors). For complete details on the use and execution of this protocol, please refer to Saito-Diaz et al. (2021).

SUBMITTER: Saito-Diaz K 

PROVIDER: S-EPMC8938318 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature