Morphological and Molecular Characterization of Paragonimus skrjabini Complex from Yunnan, China: A Brief Report.

ABSTRACT:

Purpose

To perform environmental sampling and molecular identification of Paragonimus in endemic regions, which may help in minimizing transmission among humans.

Methods

Mountain crabs from the genus Potamiscus were collected and the encysted metacercariae were extracted and subjected to morphological identification, followed by animal inoculation in Sprague-Dawley (SD) rats. After 112 days of infection, animals were killed and adult worms were extracted from lungs and muscles. The morphology of adult worms was characterized by microscopy and molecular identification was done by polymerase chain reaction, followed by sequencing of cox1 and ITS2 genes. Phylogenetic analysis was done by maximum parsimony method.

Results

A total of 447 crabs were captured from the stream

SUBMITTER: Shu QH 

PROVIDER: S-EPMC8938381 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature