Atomic resolution dynamics of cohesive interactions in phase-separated Nup98 FG domains.

ABSTRACT: Cohesive FG domains assemble into a condensed phase forming the selective permeability barrier of nuclear pore complexes. Nanoscopic insight into fundamental cohesive interactions has long been hampered by the sequence heterogeneity of native FG domains. We overcome this challenge by utilizing an engineered perfectly repetitive sequence and a combination of solution and magic angle spinning NMR spectroscopy. We map the dynamics of cohesive interactions in both phase-separated and soluble states at atomic resolution using TROSY for rotational correlation time (TRACT) measurements. We find that FG repeats exhibit nanosecond-range rotational correlation times and remain disordered in both states, although FRAP measurements show slow translation of phase-separated FG domains. NOESY measurement

SUBMITTER: Najbauer EE 

PROVIDER: S-EPMC8938434 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature