Systematic In Vivo Characterization of Fluorescent Protein Maturation in Budding Yeast.

ABSTRACT: Fluorescent protein (FP) maturation can limit the accuracy with which dynamic intracellular processes are captured and reduce the in vivo brightness of a given FP in fast-dividing cells. The knowledge of maturation timescales can therefore help users determine the appropriate FP for each application. However, in vivo maturation rates can greatly deviate from in vitro estimates that are mostly available. In this work, we present the first systematic study of in vivo maturation for 12 FPs in budding yeast. To overcome the technical limitations of translation inhibitors commonly used to study FP maturation, we implemented a new approach based on the optogenetic stimulations of FP expression in cells grown under constant nutrient conditions. Combining the rapid and

SUBMITTER: Guerra P 

PROVIDER: S-EPMC8938947 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature