A metabolomics pipeline for the mechanistic interrogation of the gut microbiome.

ABSTRACT: Gut microorganisms modulate host phenotypes and are associated with numerous health effects in humans, ranging from host responses to cancer immunotherapy to metabolic disease and obesity. However, difficulty in accurate and high-throughput functional analysis of human gut microorganisms has hindered efforts to define mechanistic connections between individual microbial strains and host phenotypes. One key way in which the gut microbiome influences host physiology is through the production of small molecules^1-3, yet progress in elucidating this chemical interplay has been hindered by limited tools calibrated to detect the products of anaerobic biochemistry in the gut. Here we construct a microbiome-focused, integrated mass-spectrometry pipeline to accelerate the identification o

SUBMITTER: Han S 

PROVIDER: S-EPMC8939302 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature