Genetic Foundations of Direct Ammonia Oxidation (Dirammox) to N₂ and MocR-Like Transcriptional Regulator DnfR in Alcaligenes faecalis Strain JQ135.

ABSTRACT: Ammonia oxidation is an important process in both the natural nitrogen cycle and nitrogen removal from engineered ecosystems. Recently, a new ammonia oxidation pathway termed Dirammox (direct ammonia oxidation, NH₃→NH₂OH→N₂) has been identified in Alcaligenes ammonioxydans. However, whether Dirammox is present in other microbes, as well as its genetic regulation, remains unknown. In this study, it was found that the metabolically versatile bacterium Alcaligenes faecalis strain JQ135 could efficiently convert ammonia into N₂ via NH₂OH under aerobic conditions. Genetic deletion and complementation results suggest that dnfABC is responsible for the ammonia oxidation to N₂ in this strain. Strai

SUBMITTER: Xu SQ 

PROVIDER: S-EPMC8939314 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature