A Novel Method to Create Efficient Phage Cocktails via Use of Phage-Resistant Bacteria.

ABSTRACT: The rapid antiphage mutation of pathogens is a big challenge often encountered in the application of phages in aquaculture, animal husbandry, and human disease prevention. A cocktail composed of phages with different infection strategies can better suppress the antiphage resistance of pathogens. However, randomly selecting phages with different infection strategies is time-consuming and labor intensive. Here, we verified that using a resistant pathogen quickly evolved under single phage infection, as the new host can easily obtain phages with different infection strategies. We randomly isolated two lytic phages (i.e., Va1 and Va2) that infect the opportunistic pathogen Vibrio alginolyticus. Whether they were used alone or in combination, the pathogen easily gained resistance. Using a mutat

SUBMITTER: Li C 

PROVIDER: S-EPMC8939320 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature