Molecular characterization of strawberry vein banding virus from China and the development of loop‑mediated isothermal amplification assays for their detection.

ABSTRACT: Strawberry vein banding virus (SVBV) is one of the serious viral pathogens infecting strawberry worldwide. To understand the molecular characterization of SVBV from China, complete genome sequences of sixteen SVBV isolates were cloned and sequenced. Sequence comparison showed they shared high nucleotide sequence identity (93.6-99.5%) with isolates from China and Japan (96.6-98.4%), while relatively low identity with the isolates from Canada (91.9-93.7%) and USA (85.5-85.9%). Phylogenetic analyses based on the complete genome sequence or coat protein (CP) gene showed the SVBV isolates clustered into three clades correlated with geographic distribution. Recombination analyses identified 13 recombinants and 21 recombinant events, indicating frequent and multiple recombinations in SVBV evoluti

SUBMITTER: Ren J 

PROVIDER: S-EPMC8940885 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature