Occurrence of a D-arabinose-containing complex-type free-N-glycan in the urine of cancer patients.

ABSTRACT: Urinary free-glycans are promising markers of disease. In this study, we attempted to identify novel tumor markers by focusing on neutral free-glycans in urine. Free-glycans extracted from the urine of normal subjects and cancer patients with gastric, colorectal, pancreatic and bile duct were fluorescently labeled with 2-aminopyridine. Profiles of these neutral free-glycans constructed using multidimensional high performance liquid chromatography separation were compared between normal controls and cancer patients. The analysis identified one glycan in the urine of cancer patients with a unique structure, which included a pentose residue. To reveal the glycan structure, the linkage fashion, monosaccharide species and enantiomer of the pentose were analyzed by high performance liquid chroma

SUBMITTER: Tanaka-Okamoto M 

PROVIDER: S-EPMC8941101 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature