Surfaceome analyses uncover CD98hc as an antibody drug-conjugate target in triple negative breast cancer.

ABSTRACT:

Background

Despite the incorporation of novel therapeutics, advanced triple negative breast cancer (TNBC) still represents a relevant clinical problem. Considering this, as well as the clinical efficacy of antibody-drug conjugates (ADCs), we aimed at identifying novel ADC targets that could be used to treat TNBC.

Methods

Transcriptomic analyses were performed on TNBC and normal samples from three different studies. Plasma membrane proteins of three cell lines representative of the TNBC subtype were identified by cell surface biotinylation or plasma membrane isolation, followed by analyses of cell surface proteins using the Surfaceome online tool. Immunofluorescence and western studies were used to characterize the action of a CD98hc-directed ADC, which was prepared by in hou

SUBMITTER: Montero JC 

PROVIDER: S-EPMC8941813 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature