Characterization and Demonstration of Mock Communities as Control Reagents for Accurate Human Microbiome Community Measurements.

ABSTRACT: Standardization and quality assurance of microbiome community analysis by high-throughput DNA sequencing require widely accessible and well-characterized reference materials. Here, we report on newly developed DNA and whole-cell mock communities to serve as control reagents for human gut microbiota measurements by shotgun metagenomics and 16S rRNA gene amplicon sequencing. The mock communities were formulated as near-even blends of up to 20 bacterial species prevalent in the human gut, span a wide range of genomic guanine-cytosine (GC) contents, and include multiple strains with Gram-positive type cell walls. Through a collaborative study, we carefully characterized the mock communities by shotgun metagenomics, using previously developed standardized protocols for DNA extraction and sequen

SUBMITTER: Tourlousse DM 

PROVIDER: S-EPMC8941912 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature