Gut Commensal Bacteroidetes Encode a Novel Class of Vitamin B₁₂-Binding Proteins.

ABSTRACT: Human gut commensal Bacteroidetes rely on multiple transport systems to acquire vitamin B₁₂ and related cobamides for fitness in the gut. In addition to a set of conserved transport proteins, these systems also include a diverse repertoire of additional proteins with unknown function. Here, we report the function and structural characterization of one of these proteins, BtuH, which binds vitamin B₁₂ directly via a C-terminal globular domain that has no known structural homologs. This protein is required for efficient B₁₂ transport and competitive fitness in the gut, demonstrating that members of the heterogeneous suite of accessory proteins encoded in Bacteroides cobamide transport system loci can play key roles in vitamin acquisition. IMPO

SUBMITTER: Putnam EE 

PROVIDER: S-EPMC8941943 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature