Genomic diversity and antimicrobial resistance of Prevotella species isolated from chronic lung disease airways.

ABSTRACT: Cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) are characterized by increasingly frequent acute pulmonary exacerbations that reduce life quality and length. Human airways are home to a rich polymicrobial environment, which includes members of the obligately anaerobic genus Prevotella. Despite their commonness, surprisingly little is known about the prevalence, role, genomic diversity and antimicrobial resistance (AMR) potential of Prevotella species and strains in healthy and diseased airways. Here, we used comparative genomics to develop a real-time PCR assay to permit rapid Prevotella species identification and quantification from cultures and clinical specimens. Assay specificity was validated across a panel of Prevotella and non-Pre

SUBMITTER: Webb KA 

PROVIDER: S-EPMC8942031 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature