Detection of glioblastoma intratumor heterogeneity in radiosensitivity using patient-derived neurosphere cultures.

ABSTRACT:

Purpose

Glioblastoma (GBM) is characterized by extensive clonal diversity suggesting the presence of tumor cells with varying degrees of treatment sensitivity. Radiotherapy is an integral part of glioblastoma treatment. Whether GBMs are comprised of spatially distinct cellular populations with uniform or varying degrees of radiosensitivity has not been established.

Methods

Spatially distinct regions of three GBMs (J3, J7 and J14) were resected and unique cell lines were derived from each region. DNA from cell lines, corresponding tumor fragments, and patient blood was extracted for whole exome sequencing. Variants, clonal composition, and functional implications were compared and analyzed with superFreq and IPA. Limiting dilution assays were performed on cell lines to measur

SUBMITTER: McAbee JH 

PROVIDER: S-EPMC8942292 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature