Comparative Ca²⁺ channel contributions to intracellular Ca²⁺ levels in the circadian clock.

ABSTRACT: Circadian rhythms in mammals are coordinated by the central clock in the brain, located in the suprachiasmatic nucleus (SCN). Multiple molecular and cellular signals display a circadian variation within SCN neurons, including intracellular Ca²⁺, but the mechanisms are not definitively established. SCN cytosolic Ca²⁺ levels exhibit a peak during the day, when both action potential firing and Ca²⁺ channel activity are increased, and are decreased at night, correlating with a reduction in firing rate. In this study, we employ a single-color fluorescence anisotropy reporter (FLARE), Venus FLARE-Cameleon, and polarization inverted selective-plane illumination microscopy to measure rhythmic changes in cytosolic Ca²⁺ in SCN neurons. Using this technique

SUBMITTER: Plante AE 

PROVIDER: S-EPMC8942421 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature