A two-subpopulation model that reflects heterogeneity of large dense core vesicles in exocytosis.

ABSTRACT: Exocytosis of large dense core vesicles is responsible for hormone secretion in neuroendocrine cells. The population of primed vesicles ready to release upon cell excitation demonstrates large heterogeneity. However, there are currently no models that clearly reflect such heterogeneity. Here, we develop a novel model based on single vesicle release events from amperometry recordings of PC12 cells using carbon fiber microelectrode. In this model, releasable vesicles can be grouped into two subpopulations, namely, SP1 and SP2. SP1 vesicles replenish quickly, with kinetics of ~0.0368 s^-1, but likely undergo slow fusion pore expansion (amperometric signals rise at ~2.5 pA/ms), while SP2 vesicles demonstrate slow replenishment (kinetics of ~0.0048 s^-1) but prefer fast dila

SUBMITTER: Qin N 

PROVIDER: S-EPMC8942488 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature