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Rrp14 controls rRNA transcription via facilitating the translocation of Pol5 into the nucleolus.

ABSTRACT: Rrp14 is a conserved protein that plays an important role in rRNA processing and ribosomal biogenesis. In Schizosaccharomyces pombe, the rrp14 gene is split into SPAC8C9.10 c (rrp14) and SPBC947.07 (rrp1402). Although the SPAC8C9.10 c gene is not essential for S. pombe survival, deletion of the gene causes the yeast cells to grow sick and to exhibit decreased rRNA transcription. We identified a novel Pol5 protein that physically interacts with the Rrp14 protein. Taking advantage of the Pil1 co-tethering assay, we found that Rrp14 facilitates the nucleolus translocation of Pol5, and the 7-RINAWN-12 motif of the Rrp14 protein is responsible for the interaction between Pol5 and Rrp14. Since deletion of the 7-RINAWN-12 motif affects rRNA transcription, we thus propose that Rrp14 affects rRNA transcription by facilitating the nucleolus translocation of Pol5.

SUBMITTER: Lin Z

PROVIDER: S-EPMC8942550 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Rrp14 controls rRNA transcription via facilitating the translocation of Pol5 into the nucleolus.

Lin Zhen Z Liu Haiyan H Chen Hongzhi H Cao Hongshi H Liu Xiaochang X Zhu Haichao H Zhao Lu L Chen Zhiming Z

Cell cycle (Georgetown, Tex.) 20220102 5

Rrp14 is a conserved protein that plays an important role in rRNA processing and ribosomal biogenesis. In Schizosaccharomyces pombe, the rrp14 gene is split into SPAC8C9.10 c (rrp14) and SPBC947.07 (rrp1402). Although the SPAC8C9.10 c gene is not essential for S. pombe survival, deletion of the gene causes the yeast cells to grow sick and to exhibit decreased rRNA transcription. We identified a novel Pol5 protein that physically interacts w ...[more]

PMID: 34974803

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