Development of a novel platform for recombinant protein production in Corynebacterium glutamicum on ethanol.

ABSTRACT: Corynebacterium glutamicum represents an emerging recombinant protein expression factory due to its ideal features for protein secretion, but its applicability is harmed by the lack of an autoinduction system with tight regulation and high yield. Here, we propose a new recombinant protein manufacturing platform that leverages ethanol as both a delayed carbon source and an inducer. First, we reanalysed the native inducible promoter P_ICL from the acetate uptake operon and found that its limited capacity is the result of the inadequate translation initial architecture. The two strategies of bicistronic design and ribozyme-based insulator can ensure the high activity of this promoter. Next, through transcriptional engineering that alters transcription factor binding sites (TF

SUBMITTER: Yu X 

PROVIDER: S-EPMC8942793 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature