The role of lysine palmitoylation/myristoylation in the function of the TEAD transcription factors.

ABSTRACT: The TEAD transcription factors are the most downstream elements of the Hippo pathway. Their transcriptional activity is modulated by different regulator proteins and by the palmitoylation/myristoylation of a specific cysteine residue. In this report, we show that a conserved lysine present in these transcription factors can also be acylated, probably following the intramolecular transfer of the acyl moiety from the cysteine. Using Scalloped (Sd), the Drosophila homolog of human TEAD, as a model, we designed a mutant protein (Glu352Gln^Sd) that is predominantly acylated on the lysine (Lys350^Sd). This protein binds in vitro to the three Sd regulators-Yki, Vg and Tgi-with a similar affinity as the wild type Sd, but it has a significantly higher thermal stability than Sd a

SUBMITTER: Mesrouze Y 

PROVIDER: S-EPMC8942982 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature