Impact of yellow fever virus envelope protein on wild-type and vaccine epitopes and tissue tropism.

ABSTRACT: The envelope (E) protein of flaviviruses is functionally associated with viral tissue tropism and pathogenicity. For yellow fever virus (YFV), viscerotropic disease primarily involving the liver is pathognomonic for wild-type (WT) infection. In contrast, the live-attenuated vaccine (LAV) strain 17D does not cause viscerotropic disease and reversion to virulence is associated with neurotropic disease. The relationship between structure-function of the E protein for WT strain Asibi and its LAV derivative 17D strain is poorly understood; however, changes to WT and vaccine epitopes have been associated with changes in virulence. Here, a panel of Asibi and 17D infectious clone mutants were generated with single-site mutations at the one membrane residue and each of the eight E protein amino aci

SUBMITTER: Davis EH 

PROVIDER: S-EPMC8942996 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature