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Multicolor labeling of airway neurons and analysis of parasympathetic heterogeneity.

ABSTRACT: We report subpopulations of airway parasympathetic neurons expressing substance P, neuronal nitric oxide synthase, and tyrosine hydroxylase, highlighting unexplored heterogeneity in this population. These neurotransmitter-specific subpopulations did not form intraganglionic interneurons, but rather, extended outside the ganglia, into the airways, to distant innervation targets. Our experiments demonstrate the utility of multicolor labeling to characterize airway innervation, allowing us to confirm the extensive heterogeneity of postganglionic parasympathetic neurons. These methods will facilitate future investigations of neurophysiology and neural contributions to airway disease.

SUBMITTER: Pincus AB

PROVIDER: S-EPMC8943012 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Multicolor labeling of airway neurons and analysis of parasympathetic heterogeneity.

Pincus Alexandra B AB Huang Samuel J SJ Lebold Katie M KM De La Torre Ubaldo U Proskocil Becky J BJ Drake Matthew G MG Nakai Hiroyuki H Fryer Allison D AD Jacoby David B DB

Scientific reports 20220323 1

We report subpopulations of airway parasympathetic neurons expressing substance P, neuronal nitric oxide synthase, and tyrosine hydroxylase, highlighting unexplored heterogeneity in this population. These neurotransmitter-specific subpopulations did not form intraganglionic interneurons, but rather, extended outside the ganglia, into the airways, to distant innervation targets. Our experiments demonstrate the utility of multicolor labeling to characterize airway innervation, allowing us to confi ...[more]

PMID: 35322058

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