Despite its sequence identity with canonical H4, Drosophila H4r product is enriched at specific chromatin regions.

ABSTRACT: Histone variants are different from their canonical counterparts in structure and are encoded by solitary genes with unique regulation to fulfill tissue or differentiation specific functions. A single H4 variant gene (His4r or H4r) that is located outside of the histone cluster and gives rise to a polyA tailed messenger RNA via replication-independent expression is preserved in Drosophila strains despite that its protein product is identical with canonical H4. In order to reveal information on the possible role of this alternative H4 we epitope tagged endogenous H4r and studied its spatial and temporal expression, and revealed its genome-wide localization to chromatin at the nucleosomal level. RNA and immunohistochemistry analysis of H4r expressed under its cognate regulation indicate expr

SUBMITTER: Abraham A 

PROVIDER: S-EPMC8943024 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature