A toolkit for recombinant production of seven human EGF family growth factors in active conformation.

ABSTRACT: Epidermal growth factors (EGF) play a wide range of roles in embryogenesis, skin development, immune response homeostasis. They are involved in several pathologies as well, including several cancer types, psoriasis, chronic pain and chronic kidney disease. All members share the structural EGF domain, which is responsible for receptor interaction, thereby initiating transduction of signals. EGF growth factors have intense use in fundamental research and high potential for biotechnological applications. However, due to their structural organization with three disulfide bonds, recombinant production of these factors in prokaryotic systems is not straightforward. A significant fraction usually forms inclusion bodies. For the fraction remaining soluble, misfolding and incomplete disulfide bond

SUBMITTER: Ferreira AS 

PROVIDER: S-EPMC8943033 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature