Altered proteome in translation initiation fidelity defective eIF5^G31R mutant causes oxidative stress and DNA damage.

ABSTRACT: The recognition of the AUG start codon and selection of an open reading frame (ORF) is fundamental to protein biosynthesis. Defect in the fidelity of start codon selection adversely affect proteome and have a pleiotropic effect on cellular function. Using proteomic techniques, we identified differential protein abundance in the translation initiation fidelity defective eIF5^G31R mutant that initiates translation using UUG codon in addition to the AUG start codon. Consistently, the eIF5^G31R mutant altered proteome involved in protein catabolism, nucleotide biosynthesis, lipid biosynthesis, carbohydrate metabolism, oxidation-reduction pathway, autophagy and re-programs the cellular pathways. The utilization of the upstream UUG codons by the eIF5^G31R mutation c

SUBMITTER: Ram AK 

PROVIDER: S-EPMC8943034 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature