Ontology highlight
ABSTRACT:
SUBMITTER: Phelps DS
PROVIDER: S-EPMC8943067 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Phelps David S DS Chinchilli Vernon M VM Yang Lili L Shearer Debra D Weisz Judith J Zhang Xuesheng X Floros Joanna J
Scientific reports 20220323 1
Using the Toponome Imaging System (TIS), a serial immunostainer, we studied the patterns of expression of multiple markers in alveolar macrophages (AM) from female mice lacking surfactant protein A (SP-A knockouts; KO) after "rescue" with exogenous SP-A1. We also used a 7-marker subset to compare with AM from males. AM were harvested 18 h after intrapharyngeal SP-A1 or vehicle, attached to slides, and subjected to serial immunostaining for 12 markers. Expression of the markers in each pixel of t ...[more]