Malignant astrocyte swelling and impaired glutamate clearance drive the expansion of injurious spreading depolarization foci.

ABSTRACT: Spreading depolarizations (SDs) indicate injury progression and predict worse clinical outcome in acute brain injury. We demonstrate in rodents that acute brain swelling upon cerebral ischemia impairs astroglial glutamate clearance and increases the tissue area invaded by SD. The cytotoxic extracellular glutamate accumulation (>15 µM) predisposes an extensive bulk of tissue (4-5 mm²) for a yet undescribed simultaneous depolarization (SiD). We confirm in rat brain slices exposed to osmotic stress that SiD is the pathological expansion of prior punctual SD foci (0.5-1 mm²), is associated with astrocyte swelling, and triggers oncotic neuron death. The blockade of astrocytic aquaporin-4 channels and Na⁺/K⁺/Cl^- co-transporters, or volume-re

SUBMITTER: Menyhart A 

PROVIDER: S-EPMC8943616 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature