Stimulated Raman scattering microscopy with spectral phasor analysis: applications in assessing drug-cell interactions.

ABSTRACT: Statins have displayed significant, although heterogeneous, anti-tumour activity in breast cancer disease progression and recurrence. They offer promise as a class of drugs, normally used for cardiovascular disease control, that could have a significant impact on the treatment of cancer. Understanding their mode of action and accurately assessing their efficacy on live cancer cells is an important and significant challenge. Stimulated Raman scattering (SRS) microscopy is a powerful, label-free imaging technique that can rapidly characterise the biochemical responses of live cell populations following drug treatment. Here, we demonstrate multi-wavelength SRS imaging together with spectral phasor analysis to characterise a panel of breast cancer cell lines (MCF-7, SK-BR-3 and MDA-MB-231 cell

SUBMITTER: Tipping WJ 

PROVIDER: S-EPMC8943890 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature