Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids.

ABSTRACT:

Background

Plant DNA isolation and purification is a time-consuming and laborious process relative to epithelial and viral DNA sample preparation due to the cell wall. The lysis of plant cells to free intracellular DNA normally requires high temperatures, chemical surfactants, and mechanical separation of plant tissue prior to a DNA purification step. Traditional DNA purification methods also do not aid themselves towards fieldwork due to the numerous chemical and bulky equipment requirements.

Results

In this study, intact plant tissue was coated by hydrophobic magnetic ionic liquids (MILs) and ionic liquids (ILs) and allowed to incubate under static conditions or dispersed in a suspension buffer to facilitate cell disruption and DNA extraction. The DNA-enriched MIL or IL wa

SUBMITTER: Emaus MN 

PROVIDER: S-EPMC8943943 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature