A functional promoter from the archaeon Halobacterium salinarum is also transcriptionally active in E. coli.

ABSTRACT:

Background

Archaea form a third domain of life that is distinct from Bacteria and Eukarya. So far, many scholars have elucidated considerable details about the typical promoter architectures of the three domains of life. However, a functional promoter from the archaeon Halobacterium salinarum has never been studied in Escherichia coli.

Results

This paper found that the promoter of Halobacterium salinarum showed a promoter function in Escherichia coli. This Escherichia coli promoter structure contains - 10 box, -10 box extension and - 29 elements, however, no -35 box. The - 29 element is exercised by the TATA box in archaea. And we isolated the RM10 fragment that possessed the fusion characteristics of bacteria and archaea, which was overlapped with functionality of TATA box

SUBMITTER: Liang J 

PROVIDER: S-EPMC8943987 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature