Saliva collection via capillary method may underestimate arboviral transmission by mosquitoes.

ABSTRACT:

Background

Arthropod-borne viruses (arboviruses) impose a major health and economic burden on human populations globally, with mosquitoes serving as important vectors. Measuring the ability of a mosquito population to transmit an arbovirus is important in terms of evaluating its public health risk. In the laboratory, a variety of methods are used to estimate arboviral transmission by mosquitoes, including indirect methods involving viral detection from mosquito saliva collected by forced salivation. The accuracy of indirect methods to estimate arbovirus transmission to live animal hosts has not been fully evaluated.

Methods

We compared three commonly used proxies of arboviral transmission, namely, the presence of virus in mosquito legs, in salivary glands (SG) and in saliva

SUBMITTER: Gloria-Soria A 

PROVIDER: S-EPMC8944160 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature