Quantitative Chemical Imaging of Bone Tissue for Intraoperative and Diagnostic Applications.

ABSTRACT: Bone is difficult to image using traditional histopathological methods, leading to challenges in intraoperative pathological evaluation that is critical in guiding surgical treatment, particularly in orthopedic oncology. In this study, we demonstrate that a multimodal quantitative imaging approach that combines stimulated Raman scattering (SRS) microscopy, two-photon fluorescence (TPF) microscopy, and second-harmonic generation (SHG) microscopy can provide useful diagnostic information regarding intact bone tissue fragments from surgical excision or biopsy specimens. We imaged bone samples from 17 patient cases and performed quantitative chemical and morphological analyses of both mineral and organic components of bone. Our main findings show that carbonate content combined with morphometr

SUBMITTER: Shin KS 

PROVIDER: S-EPMC8944199 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature