Technical Aspects of Coenzyme Q10 Analysis: Validation of a New HPLC-ED Method.

ABSTRACT: The biochemical measurement of the CoQ status in different tissues can be performed using HPLC with electrochemical detection (ED). Because the production of the electrochemical cells used with the Coulochem series detectors was discontinued, we aimed to standardize a new HPLC-ED method with new equipment. We report all technical aspects, troubleshooting and its performance in different biological samples, including plasma, skeletal muscle homogenates, urine and cultured skin fibroblasts. Analytical variables (intra- and inter-assay precision, linearity, analytical measurement range, limit of quantification, limit of detection and accuracy) were validated in calibrators and plasma samples and displayed adequate results. The comparison of the results of a new ERNDIM external quality control

SUBMITTER: Paredes-Fuentes AJ 

PROVIDER: S-EPMC8944485 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature