M⁶A Methylases Regulate Myoblast Proliferation, Apoptosis and Differentiation.

ABSTRACT: N⁶-methyladenosine (m⁶A) plays an important role in regulating gene expression. Previous studies found that m⁶A methylation affects skeletal muscle development. However, the effect of m⁶A methylases on bovine skeletal myogenesis is still unclear. Here, we found that the expression of m⁶A demethylases (FTO and ALKBH5) was significantly higher in the longissimus dorsi muscle of adult cattle than in newborn cattle. In contrast, the expression of m⁶A methyltransferases (METTL3, METTL14 and WTAP) was reduced. The mRNA expression of all five genes was found to be increased during the myogenesis of myoblasts in vitro. Knockdown of FTO or METTL3 promoted myoblast proliferation, inhibited myo

SUBMITTER: Yang X 

PROVIDER: S-EPMC8944832 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature