Ribosomal RNA 2'-O-methylations regulate translation by impacting ribosome dynamics.

ABSTRACT: SignificanceThe presence of RNA chemical modifications has long been known, but their precise molecular consequences remain unknown. 2'-O-methylation is an abundant modification that exists in RNA in all domains of life. Ribosomal RNA (rRNA) represents a functionally important RNA that is heavily modified by 2'-O-methylations. Although abundant at functionally important regions of the rRNA, the contribution of 2'-O-methylations to ribosome activities is unknown. By establishing a method to disturb rRNA 2'-O-methylation patterns, we show that rRNA 2'-O-methylations affect the function and fidelity of the ribosome and change the balance between different ribosome conformational states. Our work links 2'-O-methylation to ribosome dynamics and defines

SUBMITTER: Khoshnevis S 

PROVIDER: S-EPMC8944910 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature