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Ribosomal RNA 2'-O-methylations regulate translation by impacting ribosome dynamics.

ABSTRACT: SignificanceThe presence of RNA chemical modifications has long been known, but their precise molecular consequences remain unknown. 2'-O-methylation is an abundant modification that exists in RNA in all domains of life. Ribosomal RNA (rRNA) represents a functionally important RNA that is heavily modified by 2'-O-methylations. Although abundant at functionally important regions of the rRNA, the contribution of 2'-O-methylations to ribosome activities is unknown. By establishing a method to disturb rRNA 2'-O-methylation patterns, we show that rRNA 2'-O-methylations affect the function and fidelity of the ribosome and change the balance between different ribosome conformational states. Our work links 2'-O-methylation to ribosome dynamics and defines a set of critical rRNA 2'-O-methylations required for ribosome biogenesis and others that are dispensable.

SUBMITTER: Khoshnevis S

PROVIDER: S-EPMC8944910 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Ribosomal RNA 2'-O-methylations regulate translation by impacting ribosome dynamics.

Khoshnevis Sohail S Dreggors-Walker R Elizabeth RE Marchand Virginie V Motorin Yuri Y Ghalei Homa H

Proceedings of the National Academy of Sciences of the United States of America 20220316 12

SignificanceThe presence of RNA chemical modifications has long been known, but their precise molecular consequences remain unknown. 2'-O-methylation is an abundant modification that exists in RNA in all domains of life. Ribosomal RNA (rRNA) represents a functionally important RNA that is heavily modified by 2'-O-methylations. Although abundant at functionally important regions of the rRNA, the contribution of 2'-O-methylations to ribosome activities is unknown. By establish ...[more]

PMID: 35294285

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Ribosomal RNA 2'-O-methylations regulate translation by impacting ribosome dynamics

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