Immuno-PET Imaging of Atherosclerotic Plaques with [⁸⁹Zr]Zr-Anti-CD40 mAb-Proof of Concept.

ABSTRACT: Non-invasive imaging of atherosclerosis can help in the identification of vulnerable plaque lesions. CD40 is a co-stimulatory molecule present on various immune and non-immune cells in the plaques and is linked to inflammation and plaque instability. We hypothesize that a ⁸⁹Zr-labeled anti-CD40 monoclonal antibody (mAb) tracer has the potential to bind to cells present in atherosclerotic lesions and that CD40 Positron Emission Tomography (PET) can contribute to the detection of vulnerable atherosclerotic plaque lesions. To study this, wild-type (WT) and ApoE^-/- mice were fed a high cholesterol diet for 14 weeks to develop atherosclerosis. Mice were injected with [⁸⁹Zr]Zr-anti-CD40 mAb and the aortic uptake was evaluated and quantified using PET/Computed Tom

SUBMITTER: Poels K 

PROVIDER: S-EPMC8944956 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature