Modular Bioreactor Design for Directed Tendon/Ligament Tissue Engineering.

ABSTRACT: Functional tissue-engineered tendons and ligaments remain to be prepared in a reproducible and scalable manner. This study evaluates an acellular 3D extracellular matrix (ECM) scaffold for tendon/ligament tissue engineering and their ability to support strain-induced gene regulation associated with the tenogenesis of cultured mesenchymal stromal cells. Preliminary data demonstrate unique gene regulation patterns compared to other scaffold forms, in particular in Wnt signaling. However, the need for a robust bioreactor system that minimizes process variation was also evident. A design control process was used to design and verify the functionality of a novel bioreactor. The system accommodates 3D scaffolds with clinically-relevant sizes, is capable of long-term culture with customizable mec

SUBMITTER: Delakowski AJ 

PROVIDER: S-EPMC8945228 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature