Oxygen-Sensitive Metalloprotein Structure Determination by Cryo-Electron Microscopy.

ABSTRACT: Metalloproteins are involved in key cell processes such as photosynthesis, respiration, and oxygen transport. However, the presence of transition metals (notably iron as a component of [Fe-S] clusters) often makes these proteins sensitive to oxygen-induced degradation. Consequently, their study usually requires strict anaerobic conditions. Although X-ray crystallography has been the method of choice for solving macromolecular structures for many years, recently electron microscopy has also become an increasingly powerful structure-solving technique. We have used our previous experience with cryo-crystallography to develop a method to prepare cryo-EM grids in an anaerobic chamber and have applied it to solve the structures of apoferritin and the 3 [Fe₄S₄]-containing py

SUBMITTER: Cherrier MV 

PROVIDER: S-EPMC8945911 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature