Differential Involvement of Arabidopsis β'-COP Isoforms in Plant Development.

ABSTRACT: Coat protein I (COPI) is necessary for intra-Golgi transport and retrograde transport from the Golgi apparatus back to the endoplasmic reticulum. The key component of the COPI coat is the coatomer complex, which is composed of seven subunits (α/β/β'/γ/δ/ε/ζ) and is recruited en bloc from the cytosol onto Golgi membranes. In mammals and yeast, α- and β'-COP WD40 domains mediate cargo-selective interactions with dilysine motifs present in canonical cargoes of COPI vesicles. In contrast to mammals and yeast, three isoforms of β'-COP (β'1-3-COP) have been identified in Arabidopsis. To understand the role of Arabidopsis β'-COP isoforms in plant biology, we have identified and characterized loss-of-function mutants of the three isoforms, and double mutants were also generated. We have found that

SUBMITTER: Sanchez-Simarro J 

PROVIDER: S-EPMC8946003 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature