Targeting Proliferating Tumor-Infiltrating Macrophages Facilitates Spatial Redistribution of CD8⁺ T Cells in Pancreatic Cancer.

ABSTRACT: Tumor-associated macrophages (TAMs) play crucial roles in cancer progression, but the contributions and regulation of different macrophage subpopulations remain unclear. Here, we report a high level of TAM infiltration in human and mouse pancreatic ductal adenocarcinoma (PDAC) models and that the targeting of proliferating F4/80+ macrophages facilitated cytotoxic CD8+ T-cell-dependent antitumor immune responses. A well-defined KPC-derived PDAC cell line and the murine Panc02 PDAC cell line were used. Treatment of PDAC-bearing mice with clodronate liposomes, an agent that chemically depletes macrophages, did not impact macrophage subpopulations in the local tumor microenvironment (TME). However, further investigation using both BrdU and Ki67 to evaluate proliferating cells showed that clodr

SUBMITTER: Yang X 

PROVIDER: S-EPMC8946118 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature